Reverse Transcription:Article Title: The sTDIF signaling peptide modulates the root stele diameter and primary metabolism to accommodate symbiotic nodulation.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial Strains Sinorhizobium meliloti Sm1021 Terpolilli et al.34; Kazmierczak et al.35 Lab# Sm1021 Sinorhizobium medicae WSM419 Terpolilli et al.34; Kazmierczak et al.35 Lab# WSM419 Escherichia coli DH5α ThermoFisher Scientific; https://www. thermofisher.com Cat# 18258012 Agrobacterium rhizogenes Arqua1 Boisson-Dernier et al.36 Lab# Arqua1 Critical Commercial Assays RNeasy kit Qiagen; https://www.qiagen.com/ Cat# 74104 Quick-RNA Miniprep Kit Zymo Research; https://www.zymoresearch.com Cat# R1055 SuperScript III Reverse Transcriptase ThermoFisher Scientific; https://www. thermofisher.com Cat# 18080044 LightCycler 480 SYBR Green I Master Roche; https://lifescience.roche.com Cat# 04887352001 Experimental Models: Organisms Medicago truncatula A17 Terpolilli et al.34; Kazmierczak et al.35 Lab# MtJemA17 Medicago truncatula R108 Tadege et al.37 Lab# MtR108 Medicago truncatula stdif (R108 background) This manuscript Lab# MtJemstdif Oligonucleotides Listed in Table S2 Eurofins; https://www.eurofinsgenomics.eu/ Table S2 Recombinant DNA pAtUBI:GUS Gautrat et al.38 Lab# GG-OEGUS psTDIF:GUS This manuscript Lab# GG- psTDIF:GUS GUS RNAi Gonzalez-Rizzo et al.39 Lab# GW-GUSRNAi MtCLE37/sTDIF-MtCLE05 RNAi This manuscript Lab# GW-CLE37/05RNAi MtCLE37/sTDIF-MtCLE06 RNAi This manuscript Lab# GW-CLE37/06RNAi Softwares XLSTAT https://www.xlstat.com/ Xlstat-Basic-v17.06 MAFFT https://mafft.cbrc.jp/alignment/software/ N/A Venny https://bioinfogp.cnb.csic.es/tools/venny/ N/A MtSSP database Gene Ontology (GO) enrichment portal https://mtsspdb.zhaolab.org/atlas-internal/3880/ transcript/goenrich N/A SRplot https://www.bioinformatics.com.cn/srplot N/A MeV https://sourceforge.net/projects/mev-tm4/files/ mev-tm4/MeV%204.9.0/ 4.9.0 version, TM4 MetaboAnalyst https://www.metaboanalyst.ca/MetaboAnalyst/ home.xhtml N/A EdgeR package Robinson et al 40; https://bioconductor.org/ version 3.24.3 R https://www.r-project.org/ N/A ggplot2 (https://ggplot2.tidyverse.org/ N/A Fiji https://imagej.net/Fiji N/A Phylogenetic.io https://phylo.io/ N/A Browser/Knowledge database LEGoo Carrère et al.41; https://lipm-browsers.toulouse. inra.fr/k/legoo/ N/A Deposited data RNAseq data BioProject; https://www.ncbi.nlm.nih.gov/ bioproject/ SRA BioProject: PRJNA1275599 ll OPEN ACCESS Current Biology 35, 1–12.e1–e4, September 22, 2025 e1 Article .. The Medicago truncatula Jemalong A17 genotype was used in this study, as well as the R108 genotype for experiments related to the stdif mutant that was obtained from the Noble foundation Tnt1 insertion collection (line NF5474; https://medicago-mutant.dasnr. okstate.edu/mutant/index.php).37 The stdif mutant was backcrossed to the WT, and primers used to genotype homozygous mutants, located upstream and downstream of the Tnt1 insertion, are listed in the Table S2.
SYBR Green Assay:Article Title: The sTDIF signaling peptide modulates the root stele diameter and primary metabolism to accommodate symbiotic nodulation.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial Strains Sinorhizobium meliloti Sm1021 Terpolilli et al.34; Kazmierczak et al.35 Lab# Sm1021 Sinorhizobium medicae WSM419 Terpolilli et al.34; Kazmierczak et al.35 Lab# WSM419 Escherichia coli DH5α ThermoFisher Scientific; https://www. thermofisher.com Cat# 18258012 Agrobacterium rhizogenes Arqua1 Boisson-Dernier et al.36 Lab# Arqua1 Critical Commercial Assays RNeasy kit Qiagen; https://www.qiagen.com/ Cat# 74104 Quick-RNA Miniprep Kit Zymo Research; https://www.zymoresearch.com Cat# R1055 SuperScript III Reverse Transcriptase ThermoFisher Scientific; https://www. thermofisher.com Cat# 18080044 LightCycler 480 SYBR Green I Master Roche; https://lifescience.roche.com Cat# 04887352001 Experimental Models: Organisms Medicago truncatula A17 Terpolilli et al.34; Kazmierczak et al.35 Lab# MtJemA17 Medicago truncatula R108 Tadege et al.37 Lab# MtR108 Medicago truncatula stdif (R108 background) This manuscript Lab# MtJemstdif Oligonucleotides Listed in Table S2 Eurofins; https://www.eurofinsgenomics.eu/ Table S2 Recombinant DNA pAtUBI:GUS Gautrat et al.38 Lab# GG-OEGUS psTDIF:GUS This manuscript Lab# GG- psTDIF:GUS GUS RNAi Gonzalez-Rizzo et al.39 Lab# GW-GUSRNAi MtCLE37/sTDIF-MtCLE05 RNAi This manuscript Lab# GW-CLE37/05RNAi MtCLE37/sTDIF-MtCLE06 RNAi This manuscript Lab# GW-CLE37/06RNAi Softwares XLSTAT https://www.xlstat.com/ Xlstat-Basic-v17.06 MAFFT https://mafft.cbrc.jp/alignment/software/ N/A Venny https://bioinfogp.cnb.csic.es/tools/venny/ N/A MtSSP database Gene Ontology (GO) enrichment portal https://mtsspdb.zhaolab.org/atlas-internal/3880/ transcript/goenrich N/A SRplot https://www.bioinformatics.com.cn/srplot N/A MeV https://sourceforge.net/projects/mev-tm4/files/ mev-tm4/MeV%204.9.0/ 4.9.0 version, TM4 MetaboAnalyst https://www.metaboanalyst.ca/MetaboAnalyst/ home.xhtml N/A EdgeR package Robinson et al 40; https://bioconductor.org/ version 3.24.3 R https://www.r-project.org/ N/A ggplot2 (https://ggplot2.tidyverse.org/ N/A Fiji https://imagej.net/Fiji N/A Phylogenetic.io https://phylo.io/ N/A Browser/Knowledge database LEGoo Carrère et al.41; https://lipm-browsers.toulouse. inra.fr/k/legoo/ N/A Deposited data RNAseq data BioProject; https://www.ncbi.nlm.nih.gov/ bioproject/ SRA BioProject: PRJNA1275599 ll OPEN ACCESS Current Biology 35, 1–12.e1–e4, September 22, 2025 e1 Article .. The Medicago truncatula Jemalong A17 genotype was used in this study, as well as the R108 genotype for experiments related to the stdif mutant that was obtained from the Noble foundation Tnt1 insertion collection (line NF5474; https://medicago-mutant.dasnr. okstate.edu/mutant/index.php).37 The stdif mutant was backcrossed to the WT, and primers used to genotype homozygous mutants, located upstream and downstream of the Tnt1 insertion, are listed in the Table S2.
Recombinant:Article Title: The sTDIF signaling peptide modulates the root stele diameter and primary metabolism to accommodate symbiotic nodulation.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial Strains Sinorhizobium meliloti Sm1021 Terpolilli et al.34; Kazmierczak et al.35 Lab# Sm1021 Sinorhizobium medicae WSM419 Terpolilli et al.34; Kazmierczak et al.35 Lab# WSM419 Escherichia coli DH5α ThermoFisher Scientific; https://www. thermofisher.com Cat# 18258012 Agrobacterium rhizogenes Arqua1 Boisson-Dernier et al.36 Lab# Arqua1 Critical Commercial Assays RNeasy kit Qiagen; https://www.qiagen.com/ Cat# 74104 Quick-RNA Miniprep Kit Zymo Research; https://www.zymoresearch.com Cat# R1055 SuperScript III Reverse Transcriptase ThermoFisher Scientific; https://www. thermofisher.com Cat# 18080044 LightCycler 480 SYBR Green I Master Roche; https://lifescience.roche.com Cat# 04887352001 Experimental Models: Organisms Medicago truncatula A17 Terpolilli et al.34; Kazmierczak et al.35 Lab# MtJemA17 Medicago truncatula R108 Tadege et al.37 Lab# MtR108 Medicago truncatula stdif (R108 background) This manuscript Lab# MtJemstdif Oligonucleotides Listed in Table S2 Eurofins; https://www.eurofinsgenomics.eu/ Table S2 Recombinant DNA pAtUBI:GUS Gautrat et al.38 Lab# GG-OEGUS psTDIF:GUS This manuscript Lab# GG- psTDIF:GUS GUS RNAi Gonzalez-Rizzo et al.39 Lab# GW-GUSRNAi MtCLE37/sTDIF-MtCLE05 RNAi This manuscript Lab# GW-CLE37/05RNAi MtCLE37/sTDIF-MtCLE06 RNAi This manuscript Lab# GW-CLE37/06RNAi Softwares XLSTAT https://www.xlstat.com/ Xlstat-Basic-v17.06 MAFFT https://mafft.cbrc.jp/alignment/software/ N/A Venny https://bioinfogp.cnb.csic.es/tools/venny/ N/A MtSSP database Gene Ontology (GO) enrichment portal https://mtsspdb.zhaolab.org/atlas-internal/3880/ transcript/goenrich N/A SRplot https://www.bioinformatics.com.cn/srplot N/A MeV https://sourceforge.net/projects/mev-tm4/files/ mev-tm4/MeV%204.9.0/ 4.9.0 version, TM4 MetaboAnalyst https://www.metaboanalyst.ca/MetaboAnalyst/ home.xhtml N/A EdgeR package Robinson et al 40; https://bioconductor.org/ version 3.24.3 R https://www.r-project.org/ N/A ggplot2 (https://ggplot2.tidyverse.org/ N/A Fiji https://imagej.net/Fiji N/A Phylogenetic.io https://phylo.io/ N/A Browser/Knowledge database LEGoo Carrère et al.41; https://lipm-browsers.toulouse. inra.fr/k/legoo/ N/A Deposited data RNAseq data BioProject; https://www.ncbi.nlm.nih.gov/ bioproject/ SRA BioProject: PRJNA1275599 ll OPEN ACCESS Current Biology 35, 1–12.e1–e4, September 22, 2025 e1 Article .. The Medicago truncatula Jemalong A17 genotype was used in this study, as well as the R108 genotype for experiments related to the stdif mutant that was obtained from the Noble foundation Tnt1 insertion collection (line NF5474; https://medicago-mutant.dasnr. okstate.edu/mutant/index.php).37 The stdif mutant was backcrossed to the WT, and primers used to genotype homozygous mutants, located upstream and downstream of the Tnt1 insertion, are listed in the Table S2.
RNA sequencing:Article Title: The sTDIF signaling peptide modulates the root stele diameter and primary metabolism to accommodate symbiotic nodulation.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial Strains Sinorhizobium meliloti Sm1021 Terpolilli et al.34; Kazmierczak et al.35 Lab# Sm1021 Sinorhizobium medicae WSM419 Terpolilli et al.34; Kazmierczak et al.35 Lab# WSM419 Escherichia coli DH5α ThermoFisher Scientific; https://www. thermofisher.com Cat# 18258012 Agrobacterium rhizogenes Arqua1 Boisson-Dernier et al.36 Lab# Arqua1 Critical Commercial Assays RNeasy kit Qiagen; https://www.qiagen.com/ Cat# 74104 Quick-RNA Miniprep Kit Zymo Research; https://www.zymoresearch.com Cat# R1055 SuperScript III Reverse Transcriptase ThermoFisher Scientific; https://www. thermofisher.com Cat# 18080044 LightCycler 480 SYBR Green I Master Roche; https://lifescience.roche.com Cat# 04887352001 Experimental Models: Organisms Medicago truncatula A17 Terpolilli et al.34; Kazmierczak et al.35 Lab# MtJemA17 Medicago truncatula R108 Tadege et al.37 Lab# MtR108 Medicago truncatula stdif (R108 background) This manuscript Lab# MtJemstdif Oligonucleotides Listed in Table S2 Eurofins; https://www.eurofinsgenomics.eu/ Table S2 Recombinant DNA pAtUBI:GUS Gautrat et al.38 Lab# GG-OEGUS psTDIF:GUS This manuscript Lab# GG- psTDIF:GUS GUS RNAi Gonzalez-Rizzo et al.39 Lab# GW-GUSRNAi MtCLE37/sTDIF-MtCLE05 RNAi This manuscript Lab# GW-CLE37/05RNAi MtCLE37/sTDIF-MtCLE06 RNAi This manuscript Lab# GW-CLE37/06RNAi Softwares XLSTAT https://www.xlstat.com/ Xlstat-Basic-v17.06 MAFFT https://mafft.cbrc.jp/alignment/software/ N/A Venny https://bioinfogp.cnb.csic.es/tools/venny/ N/A MtSSP database Gene Ontology (GO) enrichment portal https://mtsspdb.zhaolab.org/atlas-internal/3880/ transcript/goenrich N/A SRplot https://www.bioinformatics.com.cn/srplot N/A MeV https://sourceforge.net/projects/mev-tm4/files/ mev-tm4/MeV%204.9.0/ 4.9.0 version, TM4 MetaboAnalyst https://www.metaboanalyst.ca/MetaboAnalyst/ home.xhtml N/A EdgeR package Robinson et al 40; https://bioconductor.org/ version 3.24.3 R https://www.r-project.org/ N/A ggplot2 (https://ggplot2.tidyverse.org/ N/A Fiji https://imagej.net/Fiji N/A Phylogenetic.io https://phylo.io/ N/A Browser/Knowledge database LEGoo Carrère et al.41; https://lipm-browsers.toulouse. inra.fr/k/legoo/ N/A Deposited data RNAseq data BioProject; https://www.ncbi.nlm.nih.gov/ bioproject/ SRA BioProject: PRJNA1275599 ll OPEN ACCESS Current Biology 35, 1–12.e1–e4, September 22, 2025 e1 Article .. The Medicago truncatula Jemalong A17 genotype was used in this study, as well as the R108 genotype for experiments related to the stdif mutant that was obtained from the Noble foundation Tnt1 insertion collection (line NF5474; https://medicago-mutant.dasnr. okstate.edu/mutant/index.php).37 The stdif mutant was backcrossed to the WT, and primers used to genotype homozygous mutants, located upstream and downstream of the Tnt1 insertion, are listed in the Table S2.
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